MICROBODIES OF THE HYDROCARBON-ASSIMILATING YEAST CANDIDA RUGOSA

Isolation of Candida rugosa lipase isoforms

The yeast Candida rugosa is a convenient source of lipases for science and industry. Crude preparation of Candida rugosa lipase (CRL) consists of several extracellular lipases. Isoenzyme profile depends on the culture or fermentation conditions. All isoforms are coded by the lip pseudogene family; they are monomers of 534 amino acids and molecular weight of about 60 kDa. They share the same cat...

Lipolytic activity of a new yeast strain Candida rugosa CMGB-CR6 isolated from oil-polluted soil

Yeast lipases present a growing interest for the synthesis of novel compounds and development of technologies with biotechnological applications. A new yeast strain CMGB-CR6 isolated from oilpolluted soil was identified as belonging to Candida rugosa using morphological observations, the API 20C AUX and Biolog Microbial ID systems and PCR-RFLP of the 5.8S rRNA-ITS and 18S rDNA-ITS. The analysis...

The Occurrence in Yeast of Cytoplasmic Granules Which Resemble Microbodies

Microbodies and microbody-like cytoplasmic granules have been identified by ultrastructural criteria in liver and kidney cells (1, 7, 17) and in various cell types in all major plant groups (14). These or similar organelles have been shown to contain hydrogen peroxide-producing and -destroying enzymes in liver and kidney cells (2, 7-9) and in the protozoan Tetrahymena pyriformis (8). The term "...

Inhibition of Candida rugosa lipase by saponins, flavonoids and alkaloids

Production of coenzyme Q by hydrocarbon-assimilating bacteria.

(pH 7.0) in the following medium: 5% nhexadecane (Tokyo Kasei Co., Tokyo, Japan), 0.1% KH2PO4, 0.4% (NH4)2S04, 0.05% FeSO4 7Hq0, 0.05% MgS04-7H20, and 0.4% yeast extract (Difco). A total of 10.4 g of dry cells was obtained from 2 liters of culture broth in 2 days at 30 C. The CoQ fraction was extracted as described by Lavate et al. (3). A 1-ml amount of the nonsaponifiable extract was taken up ...